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KMID : 0363320030240010104
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Journal of Korean Oriental Internal Medicine
2003 Volume.24 No. 1 p.104 ~ p.112
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The Effect of Sohaphyang-won’s for Delayed Neuronal Death in Hypoxia
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Lee Ji-Hun
Yun Kyoung-Sun
Jeong Seung-Hyun
Shin Gil-Cho
Lee Won-Chul
Moon Il-Soo
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Abstract
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Objectives : The purpose of this study is to evaluate the effects of Sohaphyang-won and is to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20(E20) cortical cells of a guinea pig(Sprague Dawley).
Methods : E20 cortical cells, used in this investigation were dissociated in Neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Sohaphyang-won was added to the culture media for 72 hours. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Sohaphyang-won’s effects for neuronal death protection were evaluated by LDH assay and the mechanism was studied by Bcl-2, Bak, Bax, caspase family.
Results : This study indicates that Sohaphyang-won’s effects for neuronal death protection in hypoxia is confirmed by LDH assay by the method of Embryonic day 20(E20) cortical neuroblast.
Conclusions : Sohaphyang-won’s mechanism for neuronal death protection in hypoxia restrains inflow of cytochrome C into cellularity caused by Bcl-2 increase and reduces the caspase cascade initiator caspase-10 and the effector caspase-3.
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KEYWORD
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Sohaphyang-won, hypoxia, Bcl-2, caspase
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